found 4.5–4.9 times higher antibody concentration values in the Abbott and the Siemens assay compared with the Roche assay. At the same time, some studies reported high proportional differences between the antibody assays. Several previous studies that compared quantitative SARS-CoV-2 antibody assays found that values obtained with different assays correlated well, ,, ,. The differences between the assays suggest that the tests may yield different results when tested with the same set of sera. In addition, the Abbott, Siemens and Euroimmun assays are classical antibody assays that use antigen-coated surfaces and secondary anti-human IgG antibodies, whereas the Roche assay is a bridge immunoassay that uses two antigen molecules to measure the antibody concentration. The Abbott, Siemens and Euroimmun tests measure IgG antibodies whereas the Roche test measures high-affinity antibodies of any immunoglobulin type. In comparison, the Euroimmun Anti-SARS-CoV-2-QuantiVac-ELISA (IgG) measures antibodies against the complete S1 portion of the glycoprotein. For instance, the Siemens SARS-CoV-2 IgG (sCOVG) assay, the Abbott SARS-CoV-2 IgG II Quant assay and the Roche ElecsysT Anti-SARS-CoV-2 S test quantify antibodies against the receptor-binding domain (RBD) of the spike glycoprotein. The assays differ regarding the target antigen that is being used, the immunoglobulin classes that are being measured and the technical principle. Many quantitative SARS CoV-2 antibody assays are commercially available and in clinical use. This indicates that the concentration of spike protein binding antibodies can be used as a marker to predict the likelihood that an individual is protected from disease. It has been observed that the concentration of binding antibodies against the spike protein correlates with protection from symptomatic infection. Infection with and vaccination against SARS-CoV-2 induce an antibody response against the viral spike glycoprotein.
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |